eazyplex® Superbug published references
Screening of Klebsiella pneumoniae Isolates for Carbapenemase and Hypervirulence-Associated Genes by Combining the Eazyplex® Superbug CRE and hvKp Assays.
Antibiotics 2023, 12(6), 959; https://doi.org/10.3390/antibiotics12060959 2023
The time for the results of the eazyplex® assays ranged from 6.5 to 13 min, and the total turnaround time, including sample preparation, was less than 30 min. Diagnostics should not be restricted to carbapenem-resistant isolates. Non-carbapenem-resistant hvKp can also cause severe invasive infections, and carbapenemase-producing K. pneumoniae can acquire hypervirulence-associated genes by plasmid transfer
The eazyplex® hvKp RUO assay (Amplex Diagnostics) is a ready-to-use test strip containing lyophilized master mixes with primers for K. pneumoniae, iucC, iroC, ybt, clb, rmpA, rmpA2, and an inhibition control in each well.
The eazyplex® test results for beta-lactamase and virulence genes were confirmed. The eazyplex® hvKp, currently only available as a Research Use Only assay, may be a useful tool for the rapid identification of hvKp without significant additional workload when combined with the eazyplex® Superbug CRE assay for the detection of carbapenemases.
Open access
------------------------------------------------------------------------------------------------------------------------------------------------
The Evaluation of Eazyplex® SuperBug CRE Assay Usefulness for the Detection of ESBLs and Carbapenemases Genes Directly from Urine Samples and Positive Blood Cultures
https://doi.org/10.3390/antibiotics11020138 2022
The Eazyplex® SuperBug CRE assay can be a useful tool for a rapid and reliable identification of resistance mechanism genes in Gram-negative rods, and also directly from urine and pre-cultured blood samples.
The Eazyplex® SuperBug CRE assay is based on a loop-mediated isothermal amplification of genetic material and allows for the detection of a selection of genes encoding carbapenemases, KPC, NDM, VIM, OXA-48, OXA-181 and extended-spectrum beta-lactamases from the CTX-M-1 and CTX-M-9 groups. A total of 120 clinical specimens were included in the study. The test gave valid results for 58 (96.7%) urine samples and 57 (95.0%) positive blood cultures. ESBL and/or carbapenemase enzymes genes were detected in 56 (93.3%) urine and 55 (91.7%) blood samples, respectively. The Eazyplex® SuperBug CRE assay can be used for a rapid detection of the genes encoding the most important resistance mechanisms to beta-lactams in Gram-negative rods also without the necessity of bacterial culture
-----------------------------------------------------------------------------------------------------------------------------------------
Endemicity of OXA-23 and OXA-72 in clinical isolates of Acinetobacter baumannii from three neighboring countries in Southeast Europe
https://link.springer.com/article/10.1007/s13353-021-00612-9
------------------------------------------------------------------------------------------------------------------------------------------
Molecular Characterization and Survival of Carbapenem-Resistant Acinetobacter baumannii Isolated from Hospitalized Patients in Mostar, Bosnia and Herzegovina. DOI: 10.1089/mdr 2020
The presence of blaOXA genes encoding OXA-type carbapenemases was investigated by multiplex PCR and the Eazyplex® SuperBug Acineto system and showed 100% compatibility with the detection of acquired oxacillinases.
Evaluation of the Amplex eazyplex® SuperBug Acineto test for detection of acquired OXA and NDM carbapenemases in Acinetobacter spp
DOI: 10.1016/j.jgar 2021
In conclusion, the results show that the eazyplex® SuperBug Acineto test is a rapid (15–30 min) and reliable test for the detection of the most common carbapenemases observed in Acinetobacter spp. and for species determination of A. baumannii.
--------------------------------------------------------------------------------------------------------------------------
Rapid Detection of Genes Encoding Extended-Spectrum Beta-Lactamase and Carbapenemase in Clinical Escherichia coli Isolates with eazyplex SuperBug CRE Systemhttps://doi.org/10.1089/mdr 2019
In summary, the eSBCRE test, based on LAMP method, is a reliable, easy-to-use, and timesaving molecular system. It can be successfully used in the routine microbiological diagnostic for the rapid detection of the most common ESBL and carbapenemase genes among clinical E. coli isolates. It allows to obtain results in a shorter time compared with phenotypic or even PCR, and real-time PCR methods.
----------------------------------------------------------------------------------------------------------------------------------------------
Evaluation of eazyplex® SuperBug CRE Test for Beta-Lactamase Genes Detection in Klebsiella spp. and P. aeruginosa Strains PMCID: PMC6946722 2019Based on the results, eazyplex® SuperBug CRE test (Amplex Diagnostics) can be routinely used to detect rapidly the most important mechanisms of beta-lactam resistance in Gram-negative rods, for diagnostic.
---------------------------------------------------------------------------------------------------------
Eazyplex® SuperBug CRE system for the rapid detection of carbapenemase and extended spectrum beta-lactamase genes in gram-negative bacteria
Clin Microbiol Infect Dis, 2019 doi: 10.15761/CMID.1000164
The eazyplex® SuperBug CRE system has proved to be an easy-to-use tool for the detection of carbapenemases, as well as CTX-M-type ESBLs in only 15 minutes. The system provides information that can improve the antimicrobial therapy results, and it can be useful as an epidemiologic tool in fighting against the spread of multidrug resistant bacteria.
------------------------------------------------------------------------------------------------------------------------------------------
Direct use of eazyplex® SuperBug CRE assay from positive blood cultures in conjunction with inpatient infectious disease consulting for timely appropriate antimicrobial therapy in Escherichia coli and Klebsiella pneumoniae bloodstream infection doi: 10.2147/IDR.S206323 2019
Conclusion: Our study shows that a rapid diagnostic-driven clinical strategy allowed for early prescription of potentially effective antimicrobial therapy in BSIs caused by CTX-M ESBL- and/or KPC/VIM carbapenemase-producing Ec and Kp organisms.
------------------------------------------------------------------------------------------------------------------------------
Evaluation of a Loop-Mediated Isothermal Amplification-Based Assay for the Rapid Detection of Plasmid-Encoded Colistin Resistance Gene mcr-1 in Enterobacteriaceae Isolates doi: 10.1128/AAC.02326-16. 2017
The great advantages of the rapid molecular test were the short hands-on time for each sample (2 min), the simplicity of sample processing, and the short total turnaround time to results (∼20 min). The Genie II instrument (OptiGen Limited, Horsham, United Kingdom) is mobile and can be used for at least 4 h without a power supply.
------------------------------------------------------------------------------------------------------------------------
Extended-spectrum β-lactamase (ESBL) detection directly from urine samples with the rapid isothermal amplification-based eazyplex® SuperBug CRE assay: Proof of concept PMID: 26506282 2015
A commercially available assay (eazyplex® SuperBug CRE) detecting the most common carbapenemase and ESBL types was evaluated directly on 50 urine samples. Eazyplex® correctly detected ESBL-encoding genes in all 30 urine samples with confirmed ESBL production (sensitivity 100%). Two specimens showed invalid and one specimen false-positive results (specificity 97.9%).
--------------------------------------------------------------------------------------------------
Rapid detection of β-lactamases directly from positive blood cultures using a loop-mediated isothermal amplification (LAMP)-based assay doi: 10.1016/j.ijantimicag 2015
-------------------------------------------------------------------------------------------------------------------
Evaluation of three commercial assays for rapid detection of genes encoding clinically relevant carbapenemases in cultured bacteria
https://doi.org/10.1093/jac/dku571 May 2015
Conclusions: Commercial assays offer a reliable means of detecting bacteria with clinically significant carbapenemases. Coverage of some assays required expansion to maximize the sensitivity for OXA-48-like carbapenemases. Choice will ultimately depend on preferred gene coverage, intended throughput, cost and ability to fit into local workflows
.---------------------------------------------------------------------------------------------------
Evaluation of the eazyplex® SuperBug CRE system for rapid detection of carbapenemases and ESBLs in clinical Enterobacteriaceae isolates recovered at two Spanish hospitals doi: 10.1093/jac/dku476 2014
Conclusions: The eazyplex® SuperBug CRE system proved to be a powerful tool for the detection of different carbapenemases as well as CTX-M-type ESBLs in Enterobacteriaceae with a rapid resolution time. The test has the high-performance parameters attributable to the sensitivity and specificity already demonstrated by LAMP-based assays. These results assure the usefulness of this test for routine rapid confirmation of carbapenemase-producing Enterobacteriaceae.
--------------------------------------------------------------------------------------------------------
Evaluation of a loop-mediated isothermal amplification-based methodology to detect carbapenemase carriage in Acinetobacter clinical isolates
doi: 10.1128/AAC.03870-14 2014
The Eazyplex test system allows the amplification and detection of target genes in a single step at a constant temperature and provides highly reliable results in <15 min. The implementation of this system in routine clinical laboratories provides clinicians with early valuable information for the accurate management of patients with infections caused by carbapenem-resistant Acinetobacter species
.-----------------------------------------------------------------------------------------
Evaluation of a Loop-Mediated Isothermal Amplification-Based Assay for the Rapid Detection of Plasmid-Encoded Colistin Resistance
Gene mcr-1 in Enterobacteriaceae Isolatesdoi: 10.1128/AAC.02326-16
The test clearly distinguished mcr-1-bearing isolates from intrinsically colistin-resistant isolates.The great advantages of the rapid molecular test were the short hands-on time for each sample (2 min), the simplicity of sample processing, and the short total turnaround time to results (∼20 min).
-----------------------------------------------------------------------------------------------------------
Antibiotics 2023, 12(6), 959; https://doi.org/10.3390/antibiotics12060959 2023
The time for the results of the eazyplex® assays ranged from 6.5 to 13 min, and the total turnaround time, including sample preparation, was less than 30 min. Diagnostics should not be restricted to carbapenem-resistant isolates. Non-carbapenem-resistant hvKp can also cause severe invasive infections, and carbapenemase-producing K. pneumoniae can acquire hypervirulence-associated genes by plasmid transfer
The eazyplex® hvKp RUO assay (Amplex Diagnostics) is a ready-to-use test strip containing lyophilized master mixes with primers for K. pneumoniae, iucC, iroC, ybt, clb, rmpA, rmpA2, and an inhibition control in each well.
The eazyplex® test results for beta-lactamase and virulence genes were confirmed. The eazyplex® hvKp, currently only available as a Research Use Only assay, may be a useful tool for the rapid identification of hvKp without significant additional workload when combined with the eazyplex® Superbug CRE assay for the detection of carbapenemases.
Open access
------------------------------------------------------------------------------------------------------------------------------------------------
The Evaluation of Eazyplex® SuperBug CRE Assay Usefulness for the Detection of ESBLs and Carbapenemases Genes Directly from Urine Samples and Positive Blood Cultures
https://doi.org/10.3390/antibiotics11020138 2022
The Eazyplex® SuperBug CRE assay can be a useful tool for a rapid and reliable identification of resistance mechanism genes in Gram-negative rods, and also directly from urine and pre-cultured blood samples.
The Eazyplex® SuperBug CRE assay is based on a loop-mediated isothermal amplification of genetic material and allows for the detection of a selection of genes encoding carbapenemases, KPC, NDM, VIM, OXA-48, OXA-181 and extended-spectrum beta-lactamases from the CTX-M-1 and CTX-M-9 groups. A total of 120 clinical specimens were included in the study. The test gave valid results for 58 (96.7%) urine samples and 57 (95.0%) positive blood cultures. ESBL and/or carbapenemase enzymes genes were detected in 56 (93.3%) urine and 55 (91.7%) blood samples, respectively. The Eazyplex® SuperBug CRE assay can be used for a rapid detection of the genes encoding the most important resistance mechanisms to beta-lactams in Gram-negative rods also without the necessity of bacterial culture
-----------------------------------------------------------------------------------------------------------------------------------------
Endemicity of OXA-23 and OXA-72 in clinical isolates of Acinetobacter baumannii from three neighboring countries in Southeast Europe
https://link.springer.com/article/10.1007/s13353-021-00612-9
------------------------------------------------------------------------------------------------------------------------------------------
Molecular Characterization and Survival of Carbapenem-Resistant Acinetobacter baumannii Isolated from Hospitalized Patients in Mostar, Bosnia and Herzegovina. DOI: 10.1089/mdr 2020
The presence of blaOXA genes encoding OXA-type carbapenemases was investigated by multiplex PCR and the Eazyplex® SuperBug Acineto system and showed 100% compatibility with the detection of acquired oxacillinases.
Evaluation of the Amplex eazyplex® SuperBug Acineto test for detection of acquired OXA and NDM carbapenemases in Acinetobacter spp
DOI: 10.1016/j.jgar 2021
In conclusion, the results show that the eazyplex® SuperBug Acineto test is a rapid (15–30 min) and reliable test for the detection of the most common carbapenemases observed in Acinetobacter spp. and for species determination of A. baumannii.
--------------------------------------------------------------------------------------------------------------------------
Rapid Detection of Genes Encoding Extended-Spectrum Beta-Lactamase and Carbapenemase in Clinical Escherichia coli Isolates with eazyplex SuperBug CRE Systemhttps://doi.org/10.1089/mdr 2019
In summary, the eSBCRE test, based on LAMP method, is a reliable, easy-to-use, and timesaving molecular system. It can be successfully used in the routine microbiological diagnostic for the rapid detection of the most common ESBL and carbapenemase genes among clinical E. coli isolates. It allows to obtain results in a shorter time compared with phenotypic or even PCR, and real-time PCR methods.
----------------------------------------------------------------------------------------------------------------------------------------------
Evaluation of eazyplex® SuperBug CRE Test for Beta-Lactamase Genes Detection in Klebsiella spp. and P. aeruginosa Strains PMCID: PMC6946722 2019Based on the results, eazyplex® SuperBug CRE test (Amplex Diagnostics) can be routinely used to detect rapidly the most important mechanisms of beta-lactam resistance in Gram-negative rods, for diagnostic.
---------------------------------------------------------------------------------------------------------
Eazyplex® SuperBug CRE system for the rapid detection of carbapenemase and extended spectrum beta-lactamase genes in gram-negative bacteria
Clin Microbiol Infect Dis, 2019 doi: 10.15761/CMID.1000164
The eazyplex® SuperBug CRE system has proved to be an easy-to-use tool for the detection of carbapenemases, as well as CTX-M-type ESBLs in only 15 minutes. The system provides information that can improve the antimicrobial therapy results, and it can be useful as an epidemiologic tool in fighting against the spread of multidrug resistant bacteria.
------------------------------------------------------------------------------------------------------------------------------------------
Direct use of eazyplex® SuperBug CRE assay from positive blood cultures in conjunction with inpatient infectious disease consulting for timely appropriate antimicrobial therapy in Escherichia coli and Klebsiella pneumoniae bloodstream infection doi: 10.2147/IDR.S206323 2019
Conclusion: Our study shows that a rapid diagnostic-driven clinical strategy allowed for early prescription of potentially effective antimicrobial therapy in BSIs caused by CTX-M ESBL- and/or KPC/VIM carbapenemase-producing Ec and Kp organisms.
------------------------------------------------------------------------------------------------------------------------------
Evaluation of a Loop-Mediated Isothermal Amplification-Based Assay for the Rapid Detection of Plasmid-Encoded Colistin Resistance Gene mcr-1 in Enterobacteriaceae Isolates doi: 10.1128/AAC.02326-16. 2017
The great advantages of the rapid molecular test were the short hands-on time for each sample (2 min), the simplicity of sample processing, and the short total turnaround time to results (∼20 min). The Genie II instrument (OptiGen Limited, Horsham, United Kingdom) is mobile and can be used for at least 4 h without a power supply.
------------------------------------------------------------------------------------------------------------------------
Extended-spectrum β-lactamase (ESBL) detection directly from urine samples with the rapid isothermal amplification-based eazyplex® SuperBug CRE assay: Proof of concept PMID: 26506282 2015
A commercially available assay (eazyplex® SuperBug CRE) detecting the most common carbapenemase and ESBL types was evaluated directly on 50 urine samples. Eazyplex® correctly detected ESBL-encoding genes in all 30 urine samples with confirmed ESBL production (sensitivity 100%). Two specimens showed invalid and one specimen false-positive results (specificity 97.9%).
--------------------------------------------------------------------------------------------------
Rapid detection of β-lactamases directly from positive blood cultures using a loop-mediated isothermal amplification (LAMP)-based assay doi: 10.1016/j.ijantimicag 2015
-------------------------------------------------------------------------------------------------------------------
Evaluation of three commercial assays for rapid detection of genes encoding clinically relevant carbapenemases in cultured bacteria
https://doi.org/10.1093/jac/dku571 May 2015
Conclusions: Commercial assays offer a reliable means of detecting bacteria with clinically significant carbapenemases. Coverage of some assays required expansion to maximize the sensitivity for OXA-48-like carbapenemases. Choice will ultimately depend on preferred gene coverage, intended throughput, cost and ability to fit into local workflows
.---------------------------------------------------------------------------------------------------
Evaluation of the eazyplex® SuperBug CRE system for rapid detection of carbapenemases and ESBLs in clinical Enterobacteriaceae isolates recovered at two Spanish hospitals doi: 10.1093/jac/dku476 2014
Conclusions: The eazyplex® SuperBug CRE system proved to be a powerful tool for the detection of different carbapenemases as well as CTX-M-type ESBLs in Enterobacteriaceae with a rapid resolution time. The test has the high-performance parameters attributable to the sensitivity and specificity already demonstrated by LAMP-based assays. These results assure the usefulness of this test for routine rapid confirmation of carbapenemase-producing Enterobacteriaceae.
--------------------------------------------------------------------------------------------------------
Evaluation of a loop-mediated isothermal amplification-based methodology to detect carbapenemase carriage in Acinetobacter clinical isolates
doi: 10.1128/AAC.03870-14 2014
The Eazyplex test system allows the amplification and detection of target genes in a single step at a constant temperature and provides highly reliable results in <15 min. The implementation of this system in routine clinical laboratories provides clinicians with early valuable information for the accurate management of patients with infections caused by carbapenem-resistant Acinetobacter species
.-----------------------------------------------------------------------------------------
Evaluation of a Loop-Mediated Isothermal Amplification-Based Assay for the Rapid Detection of Plasmid-Encoded Colistin Resistance
Gene mcr-1 in Enterobacteriaceae Isolatesdoi: 10.1128/AAC.02326-16
The test clearly distinguished mcr-1-bearing isolates from intrinsically colistin-resistant isolates.The great advantages of the rapid molecular test were the short hands-on time for each sample (2 min), the simplicity of sample processing, and the short total turnaround time to results (∼20 min).
-----------------------------------------------------------------------------------------------------------
