|
RESIST-BC
Reagents kit for preparation of blood cultures before performing a RESIST test. Compatible for all the targets of our RESIST kits at the exception of OXA-163 and OXA-23 (not yet validated) |
O.K.N.V.I. RESIST‑5
The latest improved version of our long established market leading immunochromatography test. The two cassettes, allows the clear and rapid identification of OXA-48-like, KPC, NDM, VIM and IMP carbapenemases in bacterial culture. |
Advantages
- Reliable and convenient tool
- To be used with the entire RESIST range of kits (not yet validated with OXA-163 and OXA-23)
- Ready and easy to use
Procedure
Standard Preparation Procedure
Standard Preparation Procedure
Faster Optional Preparation Procedure - for detection of OXA-48 and KPC only
The processed sample is then inoculated onto RESIST-5 and interpreted normally.
Performance*
Parameter |
C-PGNB |
Sensitivity |
95.8% (85.8-99.5) |
Specificity |
100% (96.2-100) |
Positive Predictive Value |
100% |
Negative Predictive Value |
100% |
Performance* - based on the publication linked below. C-PGNB Carbapenemase Producing Gram Negative Bacilli
'The limit of detection and performances of this protocol were validated in vitro on 52 C-PGNB strains spiked on a calibrated sample suspension and confirmed in clinical settings on 144 rectal swabs sampled from patients with C-PGNB digestive colonization (n = 48) and controls (patients with extended-spectrum beta-lactamase [ESBL] colonization [n = 48] and without carbapenemase/ESBL [n = 48]). The protocol detected, with 100% sensitivity, the presence of the 15 OXA-48-, 14 KPC-, 13 NDM-, and 10 VIM-producing GNB from 103 CFU/ml. The limit of detection was 2 × 102 CFU/ml. Among the 48 C-PGNB-containing rectal swabs of the validation cohort, 46 were accurately detected. False negative were observed for 1 NDM-producing Acinetobacter baumannii strain and 1 OXA-48-producing Escherichia coli strain. The 96 control swabs were negative.'
'The limit of detection and performances of this protocol were validated in vitro on 52 C-PGNB strains spiked on a calibrated sample suspension and confirmed in clinical settings on 144 rectal swabs sampled from patients with C-PGNB digestive colonization (n = 48) and controls (patients with extended-spectrum beta-lactamase [ESBL] colonization [n = 48] and without carbapenemase/ESBL [n = 48]). The protocol detected, with 100% sensitivity, the presence of the 15 OXA-48-, 14 KPC-, 13 NDM-, and 10 VIM-producing GNB from 103 CFU/ml. The limit of detection was 2 × 102 CFU/ml. Among the 48 C-PGNB-containing rectal swabs of the validation cohort, 46 were accurately detected. False negative were observed for 1 NDM-producing Acinetobacter baumannii strain and 1 OXA-48-producing Escherichia coli strain. The 96 control swabs were negative.'
