Antimicrobial Resistance Library
Rectal Screening for Carbapenemase-producing Enterobacterales
Framework of actions to contain carbapenemase-producing Enterobacterales
UK Health Security Agency September 2022
The framework sets out a range of measures, that if implemented well, will help health and social care providers minimise the impact of CPE. These include:
• active patient admission screening of risk groups
• rapid detection of patients colonised or infected with CPE, with appropriate surveillance systems to enable ongoing monitoring
FInd out more
UK Health Security Agency September 2022
The framework sets out a range of measures, that if implemented well, will help health and social care providers minimise the impact of CPE. These include:
• active patient admission screening of risk groups
• rapid detection of patients colonised or infected with CPE, with appropriate surveillance systems to enable ongoing monitoring
FInd out more
Framework of actions to contain carbapenemase-producing Enterobacterales - 2.5 Screening swabs
UK Health Security Agency September 2022
'Rectal specimens are most sensitive for detecting the carriage of antibiotic resistant Enterobacterales (14). If a screening sample is required, the ability of the lab to detect the presence of CPE can be optimised by:
• a rectal swab, making sure faecal material and/or discolouration is visible on the swab (a stool specimen if a rectal swab is not feasible or acceptable)
• a wound swab and or a urine sample (if catheterised)'
Find out more
UK Health Security Agency September 2022
'Rectal specimens are most sensitive for detecting the carriage of antibiotic resistant Enterobacterales (14). If a screening sample is required, the ability of the lab to detect the presence of CPE can be optimised by:
• a rectal swab, making sure faecal material and/or discolouration is visible on the swab (a stool specimen if a rectal swab is not feasible or acceptable)
• a wound swab and or a urine sample (if catheterised)'
Find out more
Lateral flow immunochromatographic assay for rapid screening of faecal carriage of carbapenemase-producing Enterobacteriaceae. J Antimicrob Chemother. 2018
lateral immunochromatography performed directly from rectal swab samples could represent a cost-effective alternative with a reduced turnaround time. Read more
lateral immunochromatography performed directly from rectal swab samples could represent a cost-effective alternative with a reduced turnaround time. Read more
Reduction in the detection time of OXA-48, OXA-163 and KPC carbapenemases performed with a novel immunochromatographic lateral flow assay from 2-hour-old cultures (early cultures). F. Pasteran et al poster 27th European Congress of Clinical Microbiology and Infectious Diseases, 2017
By comparing the 40/41 Adeno-Strip test results with the whole PCR procedure, no one sample has been misdiagnosed. The high simplicity and rapidity of the method let us decide to use this diagnostic tool for our routine analysis. Read more
By comparing the 40/41 Adeno-Strip test results with the whole PCR procedure, no one sample has been misdiagnosed. The high simplicity and rapidity of the method let us decide to use this diagnostic tool for our routine analysis. Read more
Four-Hour Immunochromatographic Detection of Intestinal Carriage of Carbapenemase-Producing Enterobacteriaceae: a Validation Study
Journal of Clinical Microbiology 2021
'our protocol is a rapid, easy-to-perform, and low-cost method allowing for the accurate detection of the digestive colonization with CPE in less than 4 h after sampling without any requirement for specific equipment. In this sense, it offers advantages compared to molecular biology and overnight culture on selective plates.'
'Sensitivity and specificity for C-PGNB detection were 97.7% .... and 100% .....' Read more
Journal of Clinical Microbiology 2021
'our protocol is a rapid, easy-to-perform, and low-cost method allowing for the accurate detection of the digestive colonization with CPE in less than 4 h after sampling without any requirement for specific equipment. In this sense, it offers advantages compared to molecular biology and overnight culture on selective plates.'
'Sensitivity and specificity for C-PGNB detection were 97.7% .... and 100% .....' Read more
Extended-spectrum beta-lactamases (ESBLs)
Extended-spectrum beta-lactamases (ESBLs): treatment, prevention, surveillance
How to prevent the spread of extended-spectrum beta-lactamase (ESBL) producing E. coli and Klebsiella - and treatment options..
Gov.UK 2013. Find out more
How to prevent the spread of extended-spectrum beta-lactamase (ESBL) producing E. coli and Klebsiella - and treatment options..
Gov.UK 2013. Find out more
EUCAST guidelines for detection of resistance mechanisms Version 1.0 December 2013
The recommended strategy for the detection of ESBLs in Enterobacteriaceae is based on non-susceptibility to indicator oxyiminocephalosporins, followed by phenotypic (and in some cases genotypic) confirmation tests. EUCAST 2013. Find out more
The recommended strategy for the detection of ESBLs in Enterobacteriaceae is based on non-susceptibility to indicator oxyiminocephalosporins, followed by phenotypic (and in some cases genotypic) confirmation tests. EUCAST 2013. Find out more
EUCAST guidelines for detection of resistance mechanisms Version 2.0 July 2017
Cefpodoxime is the most sensitive individual indicator cephalosporin for detection of ESBL production and may be used for screening. However, it is less specific than the combination of cefotaxime (or ceftriaxone) and ceftazidime (13, 14) and only the latter compounds are used in the confirmation testing. EUCAST 2017. Find out more
Cefpodoxime is the most sensitive individual indicator cephalosporin for detection of ESBL production and may be used for screening. However, it is less specific than the combination of cefotaxime (or ceftriaxone) and ceftazidime (13, 14) and only the latter compounds are used in the confirmation testing. EUCAST 2017. Find out more