(LDBio) Aspergillus IgG/IgM lateral flow assay is more sensitive than ImmunoCAP for CPA with or without subtle immunodeficiency
ASPERGILLUS ICT IgG-IgM
Aspergillus ICT IgG-IgM is a unitary qualitative rapid test based on immunochromatography (lateral flow), allowing the simultaneous detection of both IgG and IgM class anti-Aspergillus antibodies in human sera. Each kit is composed of the cassettes and the eluent solution that allows the chromatography. The migration is complete in 20-30 minutes. Available Packagings 20 Tests ~ LB-ASPG-AB-ICT20 |
This assay is not intended for use in invasive aspergillosis diagnosis, but for chronic forms. Antibody detection is not recommended in invasive aspergillosis diagnosis
UK Evaluations
Evaluation of the LDBio Aspergillus ICT lateral flow assay for serodiagnosis of allergic bronchopulmonary aspergillosis.
Early recognition and diagnosis of allergic bronchopulmonary aspergillosis (ABPA) is critical
to improve patient symptoms, and antifungal therapy may prevent or delay progression of
bronchiectasis and development of chronic pulmonary aspergillosis.
ABPA and control sera collected at the National Aspergillosis Centre (Manchester, UK) and/or from the Manchester Allergy, Respiratory and Thoracic Surgery research biobank were evaluated using the Aspergillus ICT assay. Results were read both visually and digitally (using a lateral flow reader). Serological Aspergillus-specific IgG and IgE, and total IgE titres were measured by ImmunoCAP.
Serological tests are a useful tool for early detection of ABPA and comprise a significant portion of the recently suggested guidelines for ABPA diagnosis [10],
In relation to Aspergillus species detected in sputum from ABPA patients (Table 3), the ICT performed well in detecting Asp antibodies for cases with A. fumigatus isolated alone (97.2% sensitivity) as well as for cases where non-A. fumigatus species were isolated in addition (95.2% sensitivity),
In this study of clinically confirmed cases of ABPA compared to diseased controls, we found the LDBio Aspergillus ICT to have good sensitivity and specificity. The test effectively distinguished between Aspergillus-sensitization complicating asthma and/or bronchiectasis, and underlying conditions. It is rapid (result in <30 minutes) and easy to perform, with simple result interpretation by visible inspection. Overall, the LDBio Aspergillus ICT exhibits excellent performance as a screening tool in the ABPA diagnostic pathway
Published: September 25, 2020 https://doi.org/10.1371/journal.pone.0238855
Evaluation of the LDBIO Aspergillus ICT lateral flow assay for serodiagnosis of Allergic Bronchopulmonary Aspergillosis.
ES Hunter, ID Page, MD Richardson, DW Denning - PloS one, 2020
Early recognition and diagnosis of allergic bronchopulmonary aspergillosis (ABPA) is critical to improve patient symptoms, and antifungal therapy may prevent or delay progression of bronchiectasis and development of chronic pulmonary aspergillosis.
Evaluation of LDBio Aspergillus ICT Lateral Flow Assay for IgG and IgM Antibody Detection in Chronic Pulmonary Aspergillosis
A total of 154 CPA patient sera collected at the National Aspergillosis Centre (Manchester, United Kingdom) and control patient sera from the Peninsula Research Bank (Exeter, United Kingdom) were evaluated.
The LDBIO Aspergillus ICT IgG-IgM immunochromatographic technology (ICT) test was compared with Aspergillus IgG titers in CPA patients, measured by ImmunoCAP-specific IgG assays (cutoff value, 40 mg of antigen-specific antibodies [mgA]/liter
For proven CPA patients versus controls, sensitivity and specificity for the LDBio Aspergillus ICT were 91.6% and 98.0%, respectively. In contrast, the routinely used ImmunoCAP assay exhibited 80.5% sensitivity for the same cohort
Of the 154 patients in our CPA case group, 108 had precipitins testing (for Aspergillus antibody) performed as part of routine diagnostics, with 57.4% sensitivity.
Our evaluation has shown very good sensitivity (91.6%) and specificity (98.0%) for diagnosis of CPA in United Kingdom patients, with the ICT test significantly outperforming our workhorse assay—the ImmunoCAP test.
Journal of Clinical Microbiology Aug 2019, 57 (9) e00538-19; DOI: 10.1128/JCM.00538-19Effect of patient immunodeficiencies on the diagnostic performance of serological assays to detect Aspergillus-specific antibodies in chronic pulmonary aspergillosis. Low or undetectable Aspergillus IgG is associated with, usually, several minor immunological defects. Aspergillus IgG/IgM lateral flow assay is more sensitive than ImmunoCAP for CPA with or without subtle immunodeficiency. CPA patients may have Aspergillus IgG detectable with different assays.
J Clin Microbiol. 2019 Aug 26;57(9):e00538-19. doi: 10.1128/JCM.00538-19.
Evaluation of the LDBio Aspergillus ICT lateral flow assay for serodiagnosis of allergic bronchopulmonary aspergillosis.
Early recognition and diagnosis of allergic bronchopulmonary aspergillosis (ABPA) is critical
to improve patient symptoms, and antifungal therapy may prevent or delay progression of
bronchiectasis and development of chronic pulmonary aspergillosis.
ABPA and control sera collected at the National Aspergillosis Centre (Manchester, UK) and/or from the Manchester Allergy, Respiratory and Thoracic Surgery research biobank were evaluated using the Aspergillus ICT assay. Results were read both visually and digitally (using a lateral flow reader). Serological Aspergillus-specific IgG and IgE, and total IgE titres were measured by ImmunoCAP.
Serological tests are a useful tool for early detection of ABPA and comprise a significant portion of the recently suggested guidelines for ABPA diagnosis [10],
In relation to Aspergillus species detected in sputum from ABPA patients (Table 3), the ICT performed well in detecting Asp antibodies for cases with A. fumigatus isolated alone (97.2% sensitivity) as well as for cases where non-A. fumigatus species were isolated in addition (95.2% sensitivity),
In this study of clinically confirmed cases of ABPA compared to diseased controls, we found the LDBio Aspergillus ICT to have good sensitivity and specificity. The test effectively distinguished between Aspergillus-sensitization complicating asthma and/or bronchiectasis, and underlying conditions. It is rapid (result in <30 minutes) and easy to perform, with simple result interpretation by visible inspection. Overall, the LDBio Aspergillus ICT exhibits excellent performance as a screening tool in the ABPA diagnostic pathway
Published: September 25, 2020 https://doi.org/10.1371/journal.pone.0238855
Evaluation of the LDBIO Aspergillus ICT lateral flow assay for serodiagnosis of Allergic Bronchopulmonary Aspergillosis.
ES Hunter, ID Page, MD Richardson, DW Denning - PloS one, 2020
Early recognition and diagnosis of allergic bronchopulmonary aspergillosis (ABPA) is critical to improve patient symptoms, and antifungal therapy may prevent or delay progression of bronchiectasis and development of chronic pulmonary aspergillosis.
Evaluation of LDBio Aspergillus ICT Lateral Flow Assay for IgG and IgM Antibody Detection in Chronic Pulmonary Aspergillosis
A total of 154 CPA patient sera collected at the National Aspergillosis Centre (Manchester, United Kingdom) and control patient sera from the Peninsula Research Bank (Exeter, United Kingdom) were evaluated.
The LDBIO Aspergillus ICT IgG-IgM immunochromatographic technology (ICT) test was compared with Aspergillus IgG titers in CPA patients, measured by ImmunoCAP-specific IgG assays (cutoff value, 40 mg of antigen-specific antibodies [mgA]/liter
For proven CPA patients versus controls, sensitivity and specificity for the LDBio Aspergillus ICT were 91.6% and 98.0%, respectively. In contrast, the routinely used ImmunoCAP assay exhibited 80.5% sensitivity for the same cohort
Of the 154 patients in our CPA case group, 108 had precipitins testing (for Aspergillus antibody) performed as part of routine diagnostics, with 57.4% sensitivity.
Our evaluation has shown very good sensitivity (91.6%) and specificity (98.0%) for diagnosis of CPA in United Kingdom patients, with the ICT test significantly outperforming our workhorse assay—the ImmunoCAP test.
Journal of Clinical Microbiology Aug 2019, 57 (9) e00538-19; DOI: 10.1128/JCM.00538-19Effect of patient immunodeficiencies on the diagnostic performance of serological assays to detect Aspergillus-specific antibodies in chronic pulmonary aspergillosis. Low or undetectable Aspergillus IgG is associated with, usually, several minor immunological defects. Aspergillus IgG/IgM lateral flow assay is more sensitive than ImmunoCAP for CPA with or without subtle immunodeficiency. CPA patients may have Aspergillus IgG detectable with different assays.
J Clin Microbiol. 2019 Aug 26;57(9):e00538-19. doi: 10.1128/JCM.00538-19.
Effect of patient immunodeficiencies on the diagnostic performance of serological assays to detect Aspergillus-specific antibodies in chronic pulmonary aspergillosis. Low or undetectable Aspergillus IgG is associated with, usually, several minor immunological defects. Aspergillus IgG/IgM lateral flow assay is more sensitive than ImmunoCAP for CPA with or without subtle immunodeficiency. CPA patients may have Aspergillus IgG detectable with different assays.
Open Access Published: January 11, 2021 DOI:https://doi.org/10.1016/j.rmed.2020.106290
Open Access Published: January 11, 2021 DOI:https://doi.org/10.1016/j.rmed.2020.106290
France Evaluation
Multicenter Evaluation of a Novel Immunochromatographic Test for Anti-Aspergillus IgG Detection
This novel anti-Aspergillus IgG ICT showed very good sensitivity (88.9%) and specificity (96.3%) for the diagnosis of Aspergillus diseases.
https://doi.org/10.3389/fcimb.2019.00012
Multicenter Evaluation of a Novel Immunochromatographic Test for Anti-Aspergillus IgG Detection
This novel anti-Aspergillus IgG ICT showed very good sensitivity (88.9%) and specificity (96.3%) for the diagnosis of Aspergillus diseases.
https://doi.org/10.3389/fcimb.2019.00012
Evaluation
Accuracy of parasitological and immunological tests for the screening of human schistosomiasis in immigrants and refugees from African countries: An approach with Latent Class Analysis
https://doi.org/10.1371/journal.pntd.0005593
The rapid test ICT was the most sensitive test, with the potential of being used as a single screening test for African migrants.
Accuracy of parasitological and immunological tests for the screening of human schistosomiasis in immigrants and refugees from African countries: An approach with Latent Class Analysis
https://doi.org/10.1371/journal.pntd.0005593
The rapid test ICT was the most sensitive test, with the potential of being used as a single screening test for African migrants.

Evaluation
Evaluation of a New Immunochromatography Technology Test (LDBio Diagnostics) To Detect Toxoplasma IgG and IgM: Comparison with the Routine Architect Technique.
DOI: 10.1128/JCM.01106-17
To conclude, this new test overcomes the limitations of automated screening techniques, which are not sensitive enough for IgG and lack specificity for IgM (rare IgM false-positive cases).
Evaluation of a New Immunochromatography Technology Test (LDBio Diagnostics) To Detect Toxoplasma IgG and IgM: Comparison with the Routine Architect Technique.
DOI: 10.1128/JCM.01106-17
To conclude, this new test overcomes the limitations of automated screening techniques, which are not sensitive enough for IgG and lack specificity for IgM (rare IgM false-positive cases).
LDBIO products