SPECIMEN PREPARATION PROCEDURE (Outline):
1 Rehydrate the CProBE selective mix and transfer measured amount to the CProBE broth.
2 Take 300 μL of the patient’s rectal swab transport medium and add to the CProBE selective broth - vortex.
3 Incubate ina stirring  water bath at 37C for 3 hours.

​4. Transfer 1.5-mL of the culture broth to a
microcentrifuge tube and.centrifuge for 10 min at 10,000 g.
5. Discard the supernatant and add 12 drops or 320 μL of LY-A buffer(provided in the RESIST rapid test kits)..

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6. Resuspend the pellet and pipette 100 μL to perform each RESIST test according to the manufacturer's recommendations.
Allow to react for 15 minutes maximum and read the result

Performance

RESIST-5 plus ReSCape was evaluated in two University Hospitals, one in France and one in Belgium.

Rectal swabs from 88 CRE colonised patients and 178 non-colonised patients were put through the RESIST + ReScape process and results compared with those obtained using reference molecular methods.

Sensitivity	​93.2%
Specificity	100%
PPV	100%
NPV	96.7%
Agreement	97.7%

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