The eazyPlex Superbug series
The WHO has declared that AMR is one of the top 10 global public health threats facing humanity. The misuse and overuse of antimicrobials are the main drivers in the development of drug resistant pathogens.
'The eazyplex® SuperBug CRE assay can be a useful tool for a rapid (less than 20 min) and reliable identification of resistance mechanism genes in Gram negative rods and also directly from urine and pre-cultured blood samples.'
'eazyplex® SuperBug Acineto test is a rapid (15–30 min) and reliable test for the detection of the most common carbapenemases observed in Acinetobacter spp. and for species determination of A. baumannii.'
'eazyplex® SuperBug Acineto test is a rapid (15–30 min) and reliable test for the detection of the most common carbapenemases observed in Acinetobacter spp. and for species determination of A. baumannii.'
Catalogue
The eazyplex® SuperBug series is a direct response to these challenges! The eazyplex® tests are easy-to-use and user-friendly molecular tests that detects resistance genes in under 30 minutes directly from sample material.
These rapid diagnostic tests cover both prevalent Gram negative resistances (such as KPC, NDM, and CTX-M) and the less prevalent yet equally important resistance genes like GIM, GES, and AmpC. The simplicity and efficiency of our tests make them almost as convenient as Point-of-Care Testing (POCT), revolutionising the way antimicrobial resistance is managed in clinical settings.
These rapid diagnostic tests cover both prevalent Gram negative resistances (such as KPC, NDM, and CTX-M) and the less prevalent yet equally important resistance genes like GIM, GES, and AmpC. The simplicity and efficiency of our tests make them almost as convenient as Point-of-Care Testing (POCT), revolutionising the way antimicrobial resistance is managed in clinical settings.
Gram negative AST - Amplex has developed the eazyplex® SuperBug range to give wider faster coverage.Resistance to antibiotics in Gram negative pathogens is nothing new, but our emphasise must be on rapid testing to support clinicians in antimicrobial stewardship.
Antibiotic Resistant Gram-negative bacteria, testing directly from:
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Nasal, throat, rectal, wound, vaginal and urethral swabs |
Bacterial culture on agar plates |
Grown 'positive' blood culture liquid media |
Urine sample |
eazyplex® CRE provides direct confirmation from a positive blood culture in 15 minutes, it will also give results from rectal swabs, positive blood culture and urine in 20 minutes or under. Both carbapenemases and ESBLs are included. We should also be aware of new threats just round the corner. SuperBug tests are available to cover less common resistances like common resistance genes such as GIM, GES and AmpC genotypes CMYII , DHA and MOX.
Key Characteristics
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The eazyplex® SuperBug series is a molecular identification product based on LAMP technology.
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Resistance to antibiotics in pathogens is widely covered, particularly in Gram-negatives. The eazyplex® SuperBug series not only cover the common resistances such as KPC, NDM, AmpC and CTX-M, but cover resistance genes, such as GIM and GES which are still rare at the moment. The Blood Screen panel not only detects the most common bacterial pathogens of sepsis but also associated CTX-M-1 and CTX -M-9 genes.
Acinetobacter: Multi-drug-resistant Acinetobacter baumannii is considered a major public-health threat owing to limited treatment options. Results of a published evaluation show that the eazyplex® SuperBug Acineto test is a rapid (15–30 min) and reliable test for the detection of the most common carbapenemases observed in Acinetobacter spp. and for species determination of A. baumannii. |
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Hands-on-time approx. 3 min.
All signals are displayed in real time during the amplification.
The eazyplex® test system is a lyophilised, ready-to-use amplification system and can be stored directly at the workplace at room temperature.
- First signals in less than 10 min.
- Complete test run finished in 20 min.
- Results output as PDF, CSV and adhesive label
All signals are displayed in real time during the amplification.
The eazyplex® test system is a lyophilised, ready-to-use amplification system and can be stored directly at the workplace at room temperature.
A short History
These publications of eazyplex®SuperBug series and the other eazyplex® panels can be accessed here
2015
Evaluation of three commercial assays for rapid detection of genes encoding clinically relevant carbapenemases in cultured bacteria. This publication came from Public Health England in 2015. doi:10.1093/jac/dku571 ''Commercial assays offer a reliable means of detecting bacteria with clinically significant carbapenemases. Coverage of some assays required expansion to maximize the sensitivity for OXA-48-like carbapenemases. Choice will ultimately depend on preferred gene coverage, intended throughput, cost and ability to fit into local workflows.''
Extended-spectrum β-lactamase (ESBL) detection directly from urine samples with the rapid isothermal amplification-based eazyplex® SuperBug CRE assay: Proof of concept. from University Hospital Basel, 2015 https://pubmed.ncbi.nlm.nih.gov/26506282/ ''A commercially available assay (eazyplex® SuperBug CRE) detecting the most common carbapenemase and ESBL types was evaluated directly on 50 urine samples. Eazyplex® correctly detected ESBL-encoding genes in all 30 urine samples with confirmed ESBL production (sensitivity 100%). Two specimens showed invalid and one specimen false-positive results (specificity 97.9%).''
2021
Evaluation of the Amplex eazyplex® SuperBug Acineto test for detection of acquired OXA and NDM carbapenemases in Acinetobacter spp from Detection of Antimicrobial Resistance Unit, University Hospital of North Norway, Tromsø. DOI:10.1016/j.jhin.2021.09.015 ''In conclusion, the results show that the eazyplex® SuperBug Acineto test is a rapid (15–30 min) and reliable test for the detection of the most common carbapenemases observed in Acinetobacter spp. and for species determination of A. baumannii.. The eazyplex® SuperBug Acineto test correctly identified all carbapenemase genes, including in strains co-harbouring two targeted acquired carbapenemase genes compared on par with the WGS results. No false-positive reactions were observed.''
2021 Endemicity of OXA-23 and OXA-72 in clinical isolates of Acinetobacter baumannii from three neighbouring countries in Southeast Europe.various Institutes in Croatia, Herzegovina and Serbia. PMID: 33502723
2022
The Evaluation of Eazyplex® SuperBug CRE Assay Usefulness for the Detection of ESBLs and Carbapenemases Genes Directly from Urine Samples and Positive Blood Cultures. from Nicolaus Copernicus University, Poland. ''The Eazyplex® SuperBug CRE assay is based on a loop-mediated isothermal amplification of genetic material and allows for the detection of a selection of genes encoding carbapenemases, KPC, NDM, VIM, OXA-48, OXA-181 and extended-spectrum beta-lactamases from the CTX-M-1 and CTX-M-9 groups. A total of 120 clinical specimens were included in the study. The test gave valid results for 58 (96.7%) urine samples and 57 (95.0%) positive blood cultures. ESBL and/or carbapenemase enzymes genes were detected in 56 (93.3%) urine and 55 (91.7%) blood samples, respectively. The Eazyplex® SuperBug CRE assay can be used for a rapid detection of the genes encoding the most important resistance mechanisms to beta-lactams in Gram-negative rods also without the necessity of bacterial culture''
https://doi.org/10.3390/antibiotics11020138
2023
Screening of Klebsiella pneumoniae Isolates for Carbapenemase and Hypervirulence-Associated Genes by Combining the Eazyplex® Superbug CRE and hvKp Assays. from Jena University Hospital, Robert Koch Institute, and Sophien Klinikum, Germany https://doi.org/10.3390/antibiotics12060959 'The time for the results of the eazyplex® assays ranged from 6.5 to 13 min, and the total turnaround time, including sample preparation, was less than 30 min.''hypervirulent K. pneumoniae (hvKp) - Diagnostics should not be restricted to carbapenem-resistant isolates. Non-carbapenem-resistant hvKp can also cause severe invasive infections, and carbapenemase-producing K. pneumoniae can acquire hypervirulence-associated genes by plasmid transfer.The eazyplex® test results for beta-lactamase and virulence genes were confirmed. The eazyplex® hvKp, currently only available as a Research Use Only assay, may be a useful tool for the rapid identification of hvKp without significant additional workload when combined with the eazyplex® Superbug CRE assay for the detection of carbapenemases
2015
Evaluation of three commercial assays for rapid detection of genes encoding clinically relevant carbapenemases in cultured bacteria. This publication came from Public Health England in 2015. doi:10.1093/jac/dku571 ''Commercial assays offer a reliable means of detecting bacteria with clinically significant carbapenemases. Coverage of some assays required expansion to maximize the sensitivity for OXA-48-like carbapenemases. Choice will ultimately depend on preferred gene coverage, intended throughput, cost and ability to fit into local workflows.''
Extended-spectrum β-lactamase (ESBL) detection directly from urine samples with the rapid isothermal amplification-based eazyplex® SuperBug CRE assay: Proof of concept. from University Hospital Basel, 2015 https://pubmed.ncbi.nlm.nih.gov/26506282/ ''A commercially available assay (eazyplex® SuperBug CRE) detecting the most common carbapenemase and ESBL types was evaluated directly on 50 urine samples. Eazyplex® correctly detected ESBL-encoding genes in all 30 urine samples with confirmed ESBL production (sensitivity 100%). Two specimens showed invalid and one specimen false-positive results (specificity 97.9%).''
2021
Evaluation of the Amplex eazyplex® SuperBug Acineto test for detection of acquired OXA and NDM carbapenemases in Acinetobacter spp from Detection of Antimicrobial Resistance Unit, University Hospital of North Norway, Tromsø. DOI:10.1016/j.jhin.2021.09.015 ''In conclusion, the results show that the eazyplex® SuperBug Acineto test is a rapid (15–30 min) and reliable test for the detection of the most common carbapenemases observed in Acinetobacter spp. and for species determination of A. baumannii.. The eazyplex® SuperBug Acineto test correctly identified all carbapenemase genes, including in strains co-harbouring two targeted acquired carbapenemase genes compared on par with the WGS results. No false-positive reactions were observed.''
2021 Endemicity of OXA-23 and OXA-72 in clinical isolates of Acinetobacter baumannii from three neighbouring countries in Southeast Europe.various Institutes in Croatia, Herzegovina and Serbia. PMID: 33502723
2022
The Evaluation of Eazyplex® SuperBug CRE Assay Usefulness for the Detection of ESBLs and Carbapenemases Genes Directly from Urine Samples and Positive Blood Cultures. from Nicolaus Copernicus University, Poland. ''The Eazyplex® SuperBug CRE assay is based on a loop-mediated isothermal amplification of genetic material and allows for the detection of a selection of genes encoding carbapenemases, KPC, NDM, VIM, OXA-48, OXA-181 and extended-spectrum beta-lactamases from the CTX-M-1 and CTX-M-9 groups. A total of 120 clinical specimens were included in the study. The test gave valid results for 58 (96.7%) urine samples and 57 (95.0%) positive blood cultures. ESBL and/or carbapenemase enzymes genes were detected in 56 (93.3%) urine and 55 (91.7%) blood samples, respectively. The Eazyplex® SuperBug CRE assay can be used for a rapid detection of the genes encoding the most important resistance mechanisms to beta-lactams in Gram-negative rods also without the necessity of bacterial culture''
https://doi.org/10.3390/antibiotics11020138
2023
Screening of Klebsiella pneumoniae Isolates for Carbapenemase and Hypervirulence-Associated Genes by Combining the Eazyplex® Superbug CRE and hvKp Assays. from Jena University Hospital, Robert Koch Institute, and Sophien Klinikum, Germany https://doi.org/10.3390/antibiotics12060959 'The time for the results of the eazyplex® assays ranged from 6.5 to 13 min, and the total turnaround time, including sample preparation, was less than 30 min.''hypervirulent K. pneumoniae (hvKp) - Diagnostics should not be restricted to carbapenem-resistant isolates. Non-carbapenem-resistant hvKp can also cause severe invasive infections, and carbapenemase-producing K. pneumoniae can acquire hypervirulence-associated genes by plasmid transfer.The eazyplex® test results for beta-lactamase and virulence genes were confirmed. The eazyplex® hvKp, currently only available as a Research Use Only assay, may be a useful tool for the rapid identification of hvKp without significant additional workload when combined with the eazyplex® Superbug CRE assay for the detection of carbapenemases
Where we are today - overnight culture then test for the big 4 or 5
Where we could be now – the big 4 and key β-lactamases direct from the sample in 20 minutes
What we should be aware of – threats round the corner
OXA variants /AmpCs: ACC, CMY II, DHA, MOX / IMP IMI GES GIM
- eazyplex® Complete A, B, and C are three options that cover the most frequently occurring carbapenemases.
- Complete C delivers cover of the UK's Big 5; plus OXA181, which has been reported increasingly worldwide. Results from the culture plate are delivered in under 30 minutes.
- eazyplex® Basic covers 4 of the UK's current Big 5 plus OXA181. Results from a culture colony are delivered in under 30 minutes.
Where we could be now – the big 4 and key β-lactamases direct from the sample in 20 minutes
- eazyplex® CRE delivers direct screening from rectal swab and urine in 20 minutes, and confirmation from a positive blood culture and culture plate in 15 minutes.
- eazyplex® CRE covers; KPC, NDM, OXA48, VIM. and OXA181 plus β-lactamases CTX-M-1 and CTX-M-9.
What we should be aware of – threats round the corner
OXA variants /AmpCs: ACC, CMY II, DHA, MOX / IMP IMI GES GIM
- eazyplex® Expert is a confirmatory test for rare carbapenemases in Enterobacteriaceae, Pseudomonads, and Acinetobacter. The sample is taken from a cultured colony. Results are delivered in under 30 minutes
- eazyplex® Acineto, with this eazyplex® you can detect the OXA variants OXA51, OXA23 , OXA40 and OXA58-like, and NDM in just 15 minutes. The sample is colony from an agar plate, optimally selective media
- eazyplex® AmpC can differentiate the following AmpC groups as culture confirmation.AmpCs: ACC, CMY II, DHA, and MOX.
eazyplex® panels publications
The eazyplex® panels publications library includes over 25 publications from 9 European countries.
The eazyplex® panels publications library includes over 25 publications from 9 European countries.
To access the eazyplex® panels publications library - click on the graphic above.
The products covered are relevant to acute infectious diseases such as septicaemia, pneumonia, and meningitis, also covered are specific key pathogens such as C. auris, MRSA, C. difficile, Salmonella typhi, SARs-CoV-2, Pneumocystis jirovecii and EHEC.
The products covered are relevant to acute infectious diseases such as septicaemia, pneumonia, and meningitis, also covered are specific key pathogens such as C. auris, MRSA, C. difficile, Salmonella typhi, SARs-CoV-2, Pneumocystis jirovecii and EHEC.
