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Two toxins – toxin A and B have been implicated in the disease caused by C. difficile. Some strains also express the binary toxin (encoded by the cdtA / B genes), which damages human cells by inhibiting actin polymerisation.
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Toxin A: This toxin is classified as an enterotoxin, meaning it specifically targets the intestines, increasing intestinal permeability, which leads to the accumulation of intestinal fluid, resulting in diarrhoea. This disruption of the intestinal barrier contributes to the symptoms and severity of the infection.
Toxin B: This toxin is identified as a potent cytotoxin, meaning it has a toxic effect on cells. In cell cultures, Toxin B causes cells to lose their shape and round off, indicating significant cellular damage. This cytotoxic effect contributes to the tissue damage and inflammation seen in infections caused by C. difficile.
The role of toxin B in the development of bowel disease is unclear. However, it has been hypothesized that the two proteins act synergistically in vivo.
What is Clostridioides difficile binary toxin (CDT)?
Binary toxin (CDT) is frequently observed in Clostridium difficile strains and is associated with an increased severity of C. difficile infection (CDI). CDT belongs to the family of binary ADP-ribosylating toxins consisting of two separate toxin components: CDTa, the enzymatic ADP-ribosyltransferase that modifies actin, and CDTb, which binds to host cells and translocates CDTa into the cytosol.
Multiple clinical studies indicate an association between binary toxin genes in C. difficile and increased 30-day CDI mortality, independent of PCR ribotype.
Toxin B: This toxin is identified as a potent cytotoxin, meaning it has a toxic effect on cells. In cell cultures, Toxin B causes cells to lose their shape and round off, indicating significant cellular damage. This cytotoxic effect contributes to the tissue damage and inflammation seen in infections caused by C. difficile.
The role of toxin B in the development of bowel disease is unclear. However, it has been hypothesized that the two proteins act synergistically in vivo.
What is Clostridioides difficile binary toxin (CDT)?
Binary toxin (CDT) is frequently observed in Clostridium difficile strains and is associated with an increased severity of C. difficile infection (CDI). CDT belongs to the family of binary ADP-ribosylating toxins consisting of two separate toxin components: CDTa, the enzymatic ADP-ribosyltransferase that modifies actin, and CDTb, which binds to host cells and translocates CDTa into the cytosol.
Multiple clinical studies indicate an association between binary toxin genes in C. difficile and increased 30-day CDI mortality, independent of PCR ribotype.
An Update on Clostridioides difficile Binary Toxin https://bit.ly/3QWuWlk
Clostridioides difficile Binary Toxin (CDT) Toxin A (tcdA) and toxin B (tcdB)
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eazyplex® C. difficile
Key characteristics are:
Options range from a basic screen to a complete toxin screen. |
eazyplex® C. difficile Kit selection
With our selection of C. difficile test kits, you decide for yourself which parameters you want to test and can use the options in a budget-friendly manner.
The eazyplex test system is a freeze dried ready to use amplification reaction and can be stored direct at the workplace. During the amplification the result is shown in Real-Time.
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The Manufacturer
Amplex Diagnostics GmbH is a German-based company founded in 2002 that specialises in providing rapid and robust CE-IVD tests to aid the diagnosis of microbiological infections. One of their products was the world's first MRSA multiplex PCR test to be CE IVD-certified for testing directly from sample material.
Amplex makes the latest advanced technology available in a simple and user-friendly manner. Their eazyplex® system which can be operated intuitively, combines isothermal amplification (LAMP) with real-time detection. The eazyplex® reagents are supported by the eazyMini which enables isothermal amplification of all DNS and RNA targets in one compact device. It was specially developed for isothermal amplification assays with real-time detection based on fluorescence and enables reading of molecular diagnostics Real-time detection within a few minutes.
www.eazyplex.com
Amplex Diagnostics GmbH is a German-based company founded in 2002 that specialises in providing rapid and robust CE-IVD tests to aid the diagnosis of microbiological infections. One of their products was the world's first MRSA multiplex PCR test to be CE IVD-certified for testing directly from sample material.
Amplex makes the latest advanced technology available in a simple and user-friendly manner. Their eazyplex® system which can be operated intuitively, combines isothermal amplification (LAMP) with real-time detection. The eazyplex® reagents are supported by the eazyMini which enables isothermal amplification of all DNS and RNA targets in one compact device. It was specially developed for isothermal amplification assays with real-time detection based on fluorescence and enables reading of molecular diagnostics Real-time detection within a few minutes.
www.eazyplex.com
The Amplex eazyMini & eazyplex® open a door to a wide range of LAMP diagnostics, offering TATs under 30 minutes
Meningitis, Septicaemia, C. difficile, MRSA, Candida auris, STI, Antibiotic Resistant Gram Negatives, EHEC, Vancomycin Resistance, Pneumocytis, Salmonella
Introducing Amplex eazyplex® LAMP assays to your hospital opens the door to a wide range of molecular diagnostics.
For example in under 30 minutes the eazyplex®Blood Screen will provide first steps in your septicaemia case. The range of assays include other pathogens that all require urgent action such as meningitis, STIs, ARMs, Candida auris etc.
