Detection of Antibiotic Resistance Mechanisms
Diagnostic Kits
Simplified and easy-to-use testing
Rosco Diagnostica acknowledges the increasing need for detection of resistance mechanisms. In order to supply our customers/users with a convenient system for detection, rapid colorimetric and assembled kits with disc diffusion tablets are available. The kits are complete with ready-to-use cartridges of the necessary combined Neo-Sensitabs™ or Diatabs™ and a guide to their use.
WHY PHENOTYPIC METHODS?
Phenotypic methods are useful for detection of beta-lactamases. They are cheap and easy to perform and interpret. Molecular techniques, on the contrary, are expensive, require significant expertise and do not identify novel and emerging resistance genes or even slightly structurally modified genes.
Rosco Diagnostica acknowledges the increasing need for detection of resistance mechanisms. In order to supply our customers/users with a convenient system for detection, rapid colorimetric and assembled kits with disc diffusion tablets are available. The kits are complete with ready-to-use cartridges of the necessary combined Neo-Sensitabs™ or Diatabs™ and a guide to their use.
WHY PHENOTYPIC METHODS?
Phenotypic methods are useful for detection of beta-lactamases. They are cheap and easy to perform and interpret. Molecular techniques, on the contrary, are expensive, require significant expertise and do not identify novel and emerging resistance genes or even slightly structurally modified genes.
BETA – LACTAMASES
Rapid Colorimetric Kits (15 min-1 hour)
98022 – Rapid ESBL Screen kit: ESBLs
98023 – Rapid BLUE CARB Kit: Carbapenemases
98024 – Neo-Rapid CARB Kit: Carbapenemases
Rosco Diagnostica Rapid colorimetric Kits have the following advantages compared to competitors:
Phenotypic Methods (18 hours)
98006 – KPC and MBL Confirm Kit: Carbapenemases
98007 – AmpC Confirm Kit: AmpC Enterobacteriaceae
98008 – ESBL + AmpC Screen Kit: ESBL + AmpC
98010 – Triple disk for detection of KPC+MBL
98011 – ESBL Confirm (acc to CLSI) Kit: ESBLs
98012 – ESBL Confirm (Chrom AmpC) Kit: ESBLs
98014 – Total ESBL Confirm Kit: ESBLs
98015 – KPC, MBL and OXA-48 Confirm Kit: Carbapenemases
98016 – Total Metallo-beta-lactamase Confirm Kit: MBLs
98019 – Total ESBL+AmpC Confirm Kit: ESBL + AmpC
98025 – KPC/MBL inP.aeruginosa/Acinetobacter, version 2
OTHERS
98009 – Urin kit Privat Praksis
98017 – Detection of hVISA, GISA, VRE, Daptomycin resistance
98018 – Detection of COLISTIN resistance in Enterobacteriaceae, P. aeruginosa and Acinetobacter spp.
The kits are complete with ready-to-use cartridges of the necessary combined Neo-Sensitabs™ or Diatabs™ and a guide to their use.
Rapid Colorimetric Kits (15 min-1 hour)
98022 – Rapid ESBL Screen kit: ESBLs
98023 – Rapid BLUE CARB Kit: Carbapenemases
98024 – Neo-Rapid CARB Kit: Carbapenemases
Rosco Diagnostica Rapid colorimetric Kits have the following advantages compared to competitors:
- More rapid results
- No reagent preparation needed
- Longer shelf-life
- Stable crystalline imipenem
- Simple and fast procedure 5 min/test
- Reduced amounts of lysis buffer or no lysis buffer
- Lower cost
- Detect new genes (compared to PCR)
Phenotypic Methods (18 hours)
98006 – KPC and MBL Confirm Kit: Carbapenemases
98007 – AmpC Confirm Kit: AmpC Enterobacteriaceae
98008 – ESBL + AmpC Screen Kit: ESBL + AmpC
98010 – Triple disk for detection of KPC+MBL
98011 – ESBL Confirm (acc to CLSI) Kit: ESBLs
98012 – ESBL Confirm (Chrom AmpC) Kit: ESBLs
98014 – Total ESBL Confirm Kit: ESBLs
98015 – KPC, MBL and OXA-48 Confirm Kit: Carbapenemases
98016 – Total Metallo-beta-lactamase Confirm Kit: MBLs
98019 – Total ESBL+AmpC Confirm Kit: ESBL + AmpC
98025 – KPC/MBL inP.aeruginosa/Acinetobacter, version 2
OTHERS
98009 – Urin kit Privat Praksis
98017 – Detection of hVISA, GISA, VRE, Daptomycin resistance
98018 – Detection of COLISTIN resistance in Enterobacteriaceae, P. aeruginosa and Acinetobacter spp.
The kits are complete with ready-to-use cartridges of the necessary combined Neo-Sensitabs™ or Diatabs™ and a guide to their use.
EUCAST ESBL/AmpC Guidelines
ROSCO kits are compliant with the EUCAST guidelines. All products required by the ESBL & AmpC phenotyping algorithm are available in kit form. Interpretation software is available free of charge to ROSCO users.
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EUCAST Carbapenemase Guidelines
ROSCO kits are compliant with the EUCAST guidelines. All products required by the carbapenemase phenotyping algorithm are available in kit form. Interpretation software is available free of charge to ROSCO users.
Why Rosco Neo-Sensitabs?
Why are most Neo-Sensitabs stable at room temperature? Unlike paper discs we use the dry crystalline antimicrobial which is compacted into tablets. ASM MIcrobe - Stable Antibiotic Sensitivity Disks DOI: https://doi.org/10.1128/AAC.9.5.848 ABSTRACT 'Two methods of preparing sensitivity disks were compared for their effect on disk stability at 25 and 37 C. One method consisted of applying a solution of the antibiotic to blank disks by the conventional procedure; the second method consisted of applying the antibiotic to the disks as a suspension of crystals. Of the four β-lactam antibiotics that were studied, disks made with suspended crystals were substantially more stable than corresponding disks made by the conventional method.' |
Does stability mean better results?
A multi centre evaluation in UK hospitals showed good comparison between paper discs and Rosco tablets with 'stable' antibiotics but significantly better results obtained with Rosco tablets when the antibiotic was less stable (co-amoxyclav).
A multi centre evaluation in UK hospitals showed good comparison between paper discs and Rosco tablets with 'stable' antibiotics but significantly better results obtained with Rosco tablets when the antibiotic was less stable (co-amoxyclav).
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